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M94A0635.TXT
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1994-10-21
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Document 0635
DOCN M94A0635
TI Twin studies suggest a host cell genetic effect on HIV replication in
human monocytes/macrophages.
DT 9412
AU Chang J; Li S; Sullivan J; Kazazi F; Naif H; Cunningham T; Virology
Department, Westmead Hospital, National Centre in HIV; Research, Sydney,
Australia.
SO Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:66 (abstract no.
TB10). Unique Identifier : AIDSLINE ASHM5/94349020
AB Biological and genetic variability is a prominent feature of HIV
strains, especially in tropism, syncytial formation and replicative
capacity. Preliminary studies also suggested there were variable host
cell effects on HIV replication in monocytes. Therefore we used
monocytes from identical (ID) twins and age matched unrelated donors
(URD) of the same sex to study possible host genetic effects on HIV
replication in monocytes. In all of the 7 pairs of identical twins the
kinetics of replication (measured by extracellular HIV antigen) of 4
different blood derived HIV strains in monocytes were insignificantly
different within pairs. However marked and significant differences in
HIV kinetics occurred within 5 of the 7 unrelated donor pairs. Very
similar results were observed with monocytes from serial bleeds of the
same donor. When studied by in situ hybridization the proportion of HIV
infected monocytes also differed within two URD pairs. Six different
strains of low passage monocytotropic blood isolate of HIV were
inoculated into panels of monocytes drawn from 15 different donors and
peak extracellular HIV titres compared. HIV strains showed patterns of
either moderate or low level replication in most donor monocytes.
However there was still marked variation in peak titres in a minority of
donors. Hence differences in HIV replication kinetics between URD
monocyte pairs contrasted with congruent patterns in ID monocytes.
Although some strains of HIV grew well in monocytes of most donors there
was still marked variation in some, strongly suggesting a host cell or
genetic influence. So far no consistent genetic linkage of HIV
replication pattern with HLA types has been observed.
DE Diseases in Twins/*GENETICS Human HIV
Infections/*GENETICS/MICROBIOLOGY HLA Antigens/GENETICS In Situ
Hybridization Macrophages/*MICROBIOLOGY Monocytes/*MICROBIOLOGY
Virulence/GENETICS Virus Replication/*GENETICS MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).